Abstract
We examined the galanin-like peptide (GALP) gene expression in the arcuate nucleus (ARC) and posterior pituitary (PP) in 6- and 18-week-old male obese fa/fa rats. GALP mRNA in the ARC in fa/fa rats was significantly decreased in 6- and 18-week-old and GALP mRNA in the PP in fa/fa rats was significantly increased in 18-week-old compared to lean Fa/? rats. Insulin treatment in hyperglycemic fa/fa rats partially reversed those changes. These results suggest that the GALP gene expression in fa/fa rats might be regulated in part by leptin-independent mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arcuate Nucleus of Hypothalamus / chemistry
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Arcuate Nucleus of Hypothalamus / metabolism*
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Blood Glucose / analysis
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Galanin-Like Peptide / genetics
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Galanin-Like Peptide / metabolism*
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Gene Expression Regulation / genetics
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Hyperglycemia / metabolism
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Insulin / pharmacology
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Leptin / metabolism
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Male
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Neuropeptide Y / genetics
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Neuropeptide Y / metabolism
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Obesity / genetics
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Obesity / metabolism*
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Pituitary Gland, Posterior / chemistry
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Pituitary Gland, Posterior / metabolism*
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Pro-Opiomelanocortin / genetics
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Pro-Opiomelanocortin / metabolism
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Rats
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Rats, Mutant Strains
Substances
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Blood Glucose
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Galanin-Like Peptide
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Insulin
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Leptin
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Neuropeptide Y
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RNA, Messenger
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Pro-Opiomelanocortin