Molecular analysis of clonality in plasma cell dyscrasias

Br J Haematol. 1992 May;81(1):18-22. doi: 10.1111/j.1365-2141.1992.tb08164.x.

Abstract

It has been suggested that multiple myeloma, generally considered a neoplastic disorder of mature plasma cells, may arise from a pluripotent haemopoietic stem cell. The possibility that circulating lymphocytes derive from the same neoplastic progenitor has been tested in a large number of studies in the past few years, as proof of the interest that this subject is raising among scientists, and also of its elusiveness. We studied a group of 29 patients with plasma cell dyscrasias in order to evaluate clonality of haemopoietic cell populations. The X-linked markers hypoxantine phosphoribosyltransferase (HPRT) and phosphoglycerate kinase (PGK) disclosed no monoclonal component in seven heterozygous women. Analysis of immunoglobulin gene rearrangement with four probes showed a germline configuration in samples from 25/29 patients. Only four bone marrow samples from subjects with aggressive disease had rearranged C mu sequence; one had rearrangement of JH and C mu.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Bone Marrow / pathology
  • Cells, Cultured
  • Female
  • Gene Rearrangement
  • Hematopoietic Stem Cells / pathology
  • Heterozygote
  • Humans
  • Hypoxanthine Phosphoribosyltransferase / analysis
  • Hypoxanthine Phosphoribosyltransferase / genetics
  • Immunoglobulin G / genetics
  • Lymphocytes / pathology
  • Male
  • Middle Aged
  • Paraproteinemias / genetics*
  • Paraproteinemias / pathology*
  • Phosphoglycerate Kinase / analysis
  • Phosphoglycerate Kinase / genetics

Substances

  • Immunoglobulin G
  • Hypoxanthine Phosphoribosyltransferase
  • Phosphoglycerate Kinase