Objective: A rapid, simple and sensitive high performance liquid chromatographic method for the determination of chloramphenicol residues in muscles and viscera of the livestock and poultry and in the shrimp has been established.
Methods: The chloramphenicol in animal tissues is extracted three times by ethyl acetate in ultrasonic. The extract is dehydrated with Na2SO4 and concentrated. The residues are cleaned up by 0.5 mol/L HClO4 and n-hexane. The purified solution is detected at UV 278 nm by HPLC.
Results: The detection limit of this method is 1.6 micrograms/kg in muscles and 3.2 micrograms/kg in viscera, the recovery is from 82.1% to 96.6%, RSD is 5.3%-6.4%.
Conclusion: This method can be widely applied to animal-foods, and its recovery is high, and its detection limit is low, and it is easily popularized in countryside.