Proposal and testing of dual-beam dynamic light scattering for two-particle microrheology

Appl Opt. 2004 Jun 10;43(17):3382-90. doi: 10.1364/ao.43.003382.

Abstract

A dual-beam dynamic light-scattering arrangement is devised to measure the time-dependent mean squared relative displacement of a pair of tracer particles with a small separation of micrometers. The technique is tested by the measurement of the relative diffusion of polymer latex spheres suspended in a simple viscous fluid. The experiment verifies the theory and demonstrates its applications. The dual-beam dynamic light-scattering technique, when combined with an optical microscope, provides a powerful tool for the study of two-particle microrheology of soft materials. The advantages of the new technique are its high statistical accuracy, faster temporal response, and ease of use.