Abstract
Utilizing a screening method designed for the identification of genes involved with enhanced protein secretion in Saccharomyces cerevisiae we identified a gene, which we named PSE-1 (Protein Secretion Enhancer). Overexpression of PSE-1 in a multi-copy plasmid, as shown by Northern hybridization, gave a fourfold enhancement in total protein secretion. The repertoire of proteins that are found to be secreted in greater quantities include three known biologically active proteins: k1 killer toxin, alpha-factor, and acid phosphatase. The PSE-1 gene is located on chromosome XII of the yeast genome and codes for a hydrophobic protein containing 1089 amino acids. Haploid yeast cells that contained a LEU2 insertion mutation in PSE-1 grow very poorly, a phenotype similar to other conditional SEC mutants at restrictive temperature.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase / metabolism
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA, Fungal
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Electrophoresis, Polyacrylamide Gel
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Genes, Fungal
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Mating Factor
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Membrane Transport Proteins*
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Molecular Sequence Data
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Peptides / metabolism
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Phenotype
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Plasmids
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Receptors, Cytoplasmic and Nuclear / genetics*
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
Substances
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DNA, Fungal
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Fungal Proteins
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Membrane Transport Proteins
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PSE1 protein, S cerevisiae
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Peptides
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Receptors, Cytoplasmic and Nuclear
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Saccharomyces cerevisiae Proteins
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Mating Factor
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Acid Phosphatase
Associated data
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GENBANK/M74319
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GENBANK/M74320
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GENBANK/M74321
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GENBANK/M74322
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GENBANK/M74323
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GENBANK/M74324
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GENBANK/X66084
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GENBANK/Z11538
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GENBANK/Z15036
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GENBANK/Z15040