Flow cytometric test for beryllium sensitivity

Cytometry B Clin Cytom. 2004 Jul;60(1):23-30. doi: 10.1002/cyto.b.20015.

Abstract

Background: Chronic beryllium disease (CBD) is an occupational granulomatous disorder characterized by hypersensitivity to beryllium, mediated by CD4+ T lymphocytes, and predominantly affects the lungs. In this disorder, lymphocyte proliferative responses to beryllium, measured by 3H thymidine incorporation, are used for diagnosis of CBD, for screening asymptomatic workers or former workers to detect unrecognized disease, and for surveillance as a bioassay to detect abnormal exposures. Problems with test variability and the use of radioactivity have recently led to the search for alternative methods.

Methods: We applied a 5,6-carboxyfluorescein diacetate succinimidyl ester flow cytometric technique for measurement of mitogen- and antigen-induced T-lymphocyte proliferation to a group of beryllium-exposed sensitized individuals and beryllium-unexposed controls.

Results: We detected mitogen and antigen proliferative responses in CD3+, CD4+, and CD8+ subpopulations. Phytohemagglutinin and Candida stimulated CD4+ and CD8+ T-cell responses, but beryllium appeared to stimulate only CD3+/CD4+ responses.

Conclusions: This technique may provide a sensitive, nonradioactive alternative to the traditional proliferation tests that measure beryllium sensitivity. It offers the added specificity of enabling phenotypic description of the responding cell type and may prove to be easier to standardize for clinical use.

Publication types

  • Comparative Study

MeSH terms

  • Air Pollutants, Occupational / toxicity*
  • Berylliosis / immunology
  • Berylliosis / pathology*
  • Beryllium / toxicity*
  • CD3 Complex / immunology
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / pathology
  • CD8-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / pathology
  • Cell Proliferation / drug effects
  • Chronic Disease
  • Flow Cytometry
  • Fluoresceins
  • Fluorescent Dyes
  • Humans
  • Succinimides
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology*
  • Thymidine / metabolism

Substances

  • 5-(6)-carboxyfluorescein diacetate succinimidyl ester
  • Air Pollutants, Occupational
  • CD3 Complex
  • Fluoresceins
  • Fluorescent Dyes
  • Succinimides
  • Beryllium
  • Thymidine