Abstract
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Catalytic / pharmacology*
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Flow Cytometry / methods
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Fructose-Bisphosphate Aldolase / drug effects
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Fructose-Bisphosphate Aldolase / metabolism*
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Hybridomas
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Kinetics
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Recombinant Proteins / drug effects
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Recombinant Proteins / metabolism
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Transfection
Substances
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Antibodies, Catalytic
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Recombinant Proteins
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Fructose-Bisphosphate Aldolase