Characterization of a novel selenium methyltransferase from freshwater bacteria showing strong similarities with the calicheamicin methyltransferase

Biochim Biophys Acta. 2004 Jul 13;1679(1):80-5. doi: 10.1016/j.bbaexp.2004.05.001.

Abstract

A novel group of Se-methyltransferases is presented. The genetic determinant, named mmtA, which revealed this group was isolated from selenite and selenate-resistant freshwater bacteria. E. coli expressing mmtA and grown with a Se supplement emitted dimethyl selenide (DMSe) and dimethyl diselenide (DMDSe). Phylogenetic analysis divided MmtA-like bacterial sequences into two clusters, one grouping MmtA with S- and O-methyltransferases, and one grouping UbiE C-methyltransferases. Se methylation by some of these MmtA phyletic neighbours was investigated.

MeSH terms

  • Amino Acid Sequence
  • Bacteria / enzymology*
  • Base Sequence
  • DNA Primers
  • Gas Chromatography-Mass Spectrometry
  • Methyltransferases / chemistry
  • Methyltransferases / metabolism*
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Water Microbiology

Substances

  • DNA Primers
  • Methyltransferases

Associated data

  • GENBANK/AY569977
  • GENBANK/AY569978