Abstract
Two new mouse cell mutants, resistant to either 80 or 100 mM CdCl2, were isolated to study the regulation of transcription by the glucocorticoid hormones. Their metallothionein mt-1% and mt-2+ genes were amplified coordinately to a maximum of 30 copies per cell. By Southern blot analysis, no gross rearrangement was detectable near the mt+ loci. Contrary to other mutants previously isolated, the metallothionein-specific mRNAs of these mutants are inducible by dexamethasone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoradiography
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Blotting, Northern
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Blotting, Southern
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Cadmium / pharmacology
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Cells, Cultured
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DNA
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Dexamethasone / pharmacology*
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Electrophoresis, Polyacrylamide Gel
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Gene Amplification
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Gene Expression / drug effects
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Metallothionein / biosynthesis
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Metallothionein / genetics*
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Mice
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Mutation
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RNA, Messenger / biosynthesis
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Transcription, Genetic
Substances
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RNA, Messenger
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Cadmium
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Dexamethasone
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DNA
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Metallothionein