Phosphorylcholine-carbohydrate-protein conjugates efficiently induce hapten-specific antibodies which recognize both Streptococcus pneumoniae and Neisseria meningitidis: a potential multitarget vaccine against respiratory infections

J Med Chem. 2004 Jul 29;47(16):3916-9. doi: 10.1021/jm040783p.

Abstract

Phosphorylcholine (ChoP) is commonly expressed at the surface of pathogens of the respiratory tract, including Streptococcus pneumoniae and Neisseria meningitidis. We designed a synthetic hapten comprising ChoP and part of its native carrier structure in S. pneumoniae, i.e. N-acetyl-D-galactosamine (GalNAc). Protein conjugates of this hapten induced GalNAc-ChoP-specific antibodies which recognized ChoP on both S. pneumoniae and N. meningitidis. GalNAc-ChoP could therefore lead to the rational design of a novel multipurpose vaccine against respiratory infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylgalactosamine / chemistry*
  • Animals
  • Antibody Formation
  • Antigens, Bacterial / immunology*
  • Bacterial Vaccines / chemical synthesis
  • Bacterial Vaccines / immunology*
  • Haptens / immunology
  • Mice
  • Neisseria meningitidis / immunology*
  • Phosphorylcholine / chemistry*
  • Respiratory Tract Infections / microbiology
  • Serum Albumin / chemistry
  • Streptococcus pneumoniae / chemistry
  • Streptococcus pneumoniae / immunology*
  • Tetanus Toxin / chemistry

Substances

  • Antigens, Bacterial
  • Bacterial Vaccines
  • Haptens
  • Serum Albumin
  • Tetanus Toxin
  • Phosphorylcholine
  • Acetylgalactosamine