Abstract
Recombinant Escherichia coli strain C600/pBV-TRAIL (encoding for 114-281 amino acids of TRAIL's soluble fragment) produced a recombinant human tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Using a combined strategy of exponential feeding and pH-stat feeding, high concentrations of biomass (65 g dry wt l(-1)) and active soluble TRAIL (1.4 g l(-1)) were obtained within 30 h. The accumulation of acetate, which usually occurs during the process of high-density culture of Escherichia coli and especially in the induction stage of protein synthesis, was avoided.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis Regulatory Proteins
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Bioreactors / microbiology*
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Cell Culture Techniques / methods*
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Cell Proliferation
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Cloning, Molecular / methods
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Escherichia coli / physiology*
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Gene Expression Regulation, Bacterial / physiology
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Glucose / metabolism
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Humans
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Membrane Glycoproteins / biosynthesis*
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / isolation & purification
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Nitrogen / metabolism
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Protein Engineering / methods*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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TNF-Related Apoptosis-Inducing Ligand
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Tumor Necrosis Factor-alpha / biosynthesis*
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Tumor Necrosis Factor-alpha / chemistry
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Tumor Necrosis Factor-alpha / genetics*
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Tumor Necrosis Factor-alpha / isolation & purification
Substances
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Apoptosis Regulatory Proteins
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Membrane Glycoproteins
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Recombinant Proteins
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TNF-Related Apoptosis-Inducing Ligand
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TNFSF10 protein, human
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Tumor Necrosis Factor-alpha
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Glucose
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Nitrogen