A STAT4-dependent Th1 response is required for resistance to the helminth parasite Taenia crassiceps

Infect Immun. 2004 Aug;72(8):4552-60. doi: 10.1128/IAI.72.8.4552-4560.2004.

Abstract

To determine the role of STAT4-dependent Th1 responses in the regulation of immunity to the helminth parasite Taenia crassiceps, we monitored infections with this parasite in resistant mice lacking the STAT4 gene. While T. crassiceps-infected STAT4(+/+) mice rapidly resolved the infection, STAT4(-/-) mice were highly susceptible to infection and displayed large parasite loads. Moreover, the inability of STAT4(-/-) mice to control the infection was associated with the induction of an antigen-specific Th2-type response characterized by significantly higher levels of Th2-associated immunoglobulin G1 (IgG1) and total IgE as well as interleukin-4 (IL-4), IL-10, and IL-13 than those in STAT4(+/+) mice, who produced significantly more gamma interferon. Furthermore, early after infection, macrophages from STAT4(-/-) mice produced lower levels of the pro-inflammatory cytokines IL-12, tumor necrosis factor alpha, IL-1 beta, and nitric oxide (NO) than those from STAT4(+/+) mice, suggesting a pivotal role for macrophages in mediating protection against cysticercosis. These findings demonstrate a critical role for the STAT4 signaling pathway in the development of a Th1-type immune response that is essential for mediating protection against the larval stage of T. crassiceps infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Helminth / blood
  • Cysticercosis / immunology*
  • Cysticercosis / parasitology
  • Cysticercus / immunology
  • Cytokines / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Lymphocyte Activation
  • Macrophage Activation
  • Macrophages, Peritoneal / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Receptors, CCR5 / metabolism
  • Receptors, IgE / metabolism
  • STAT4 Transcription Factor
  • Taenia / growth & development
  • Taenia / pathogenicity*
  • Th1 Cells / immunology*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*

Substances

  • Antibodies, Helminth
  • Cytokines
  • DNA-Binding Proteins
  • Receptors, CCR5
  • Receptors, IgE
  • STAT4 Transcription Factor
  • Stat4 protein, mouse
  • Trans-Activators