Abstract
To identify the elements of two chemokines [monocyte chemoattractant protein-1 (MCP-1) and eotaxin] that control their differential recognition by their respective receptors (CCR2 and CCR3), we have studied the receptor interactions of MCP-1-eotaxin chimeras. Each receptor was found to exhibit a distinct binding preference for proteins containing the amino-terminal region of the cognate chemokine for that receptor. However, other elements dictating chemokine preference were different for the two receptors. In some cases, the influence of replacing a particular region was dependent on the identities of neighboring regions, indicating a complex network of cooperative and/or compensating interactions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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Chemokine CCL11
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Chemokine CCL2 / chemistry
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Chemokine CCL2 / genetics
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Chemokine CCL2 / metabolism*
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Chemokines / pharmacology
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Chemokines, CC / chemistry
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Chemokines, CC / genetics
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Chemokines, CC / metabolism*
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Cloning, Molecular
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Models, Molecular
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Conformation
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Receptors, Chemokine / drug effects
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Receptors, Chemokine / physiology
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Sensitivity and Specificity
Substances
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Chemokine CCL11
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Chemokine CCL2
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Chemokines
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Chemokines, CC
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Receptors, Chemokine
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Recombinant Fusion Proteins