Objective: To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae.
Methods: Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schistosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced.
Results: Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively.
Conclusion: Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.