Objective: To construct and express recombinant plasmid containing the structural gene encoding Mr 31 000 antigen of Trichinella spiralis muscle larvae.
Methods: The target gene TspE1 was amplified by RT-PCR, cloned into pUC18 vector, and sub-cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3-TspE1 by gene-gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining.
Results and conclusion: The recombinant plasmid pcDNA3-TspE1 was successfully constructed and expressed in the BALB/c mice.