Abstract
Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyleinating disease of the central nervous system that is induced in laboratory animals by the generation of an immune response against myelin epitopes. It has been used as a prototype of Th1-driven, organ-specific autoimmunity and as a model for the human disease multiple sclerosis. This chapter describes two classic protocols for EAE induction (active immunization and adoptive transfer). Subheading 3.3. describes methods for rating clinical disease in symptomatic animals. Subheading 3.4. includes instructions for the isolation of mononuclear cells from the inflamed spinal cords from mice with EAE.
MeSH terms
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Adoptive Transfer
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Amino Acid Sequence
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Animals
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Autoantigens / administration & dosage
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Autoantigens / genetics
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Encephalomyelitis, Autoimmune, Experimental / etiology*
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Encephalomyelitis, Autoimmune, Experimental / immunology
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Humans
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Mice
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Mice, Inbred Strains
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Molecular Sequence Data
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Multiple Sclerosis / etiology
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Multiple Sclerosis / immunology
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Myelin Basic Protein / administration & dosage
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Myelin Basic Protein / genetics
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Myelin Basic Protein / immunology
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Myelin Proteins
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Myelin Proteolipid Protein / administration & dosage
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Myelin Proteolipid Protein / genetics
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Myelin Proteolipid Protein / immunology
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Myelin-Associated Glycoprotein / administration & dosage
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Myelin-Associated Glycoprotein / genetics
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Myelin-Associated Glycoprotein / immunology
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Myelin-Oligodendrocyte Glycoprotein
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Peptide Fragments / administration & dosage
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Th1 Cells / immunology
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Vaccination
Substances
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Autoantigens
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MOG protein, human
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Mog protein, mouse
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Myelin Basic Protein
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Myelin Proteins
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Myelin Proteolipid Protein
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Myelin-Associated Glycoprotein
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Myelin-Oligodendrocyte Glycoprotein
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Peptide Fragments