Expression profiling of T-cell lymphomas differentiates peripheral and lymphoblastic lymphomas and defines survival related genes

Clin Cancer Res. 2004 Aug 1;10(15):4971-82. doi: 10.1158/1078-0432.CCR-04-0269.

Abstract

Purpose: T-Cell lymphomas constitute heterogeneous and aggressive tumors in which pathogenic alterations remain largely unknown. Expression profiling has demonstrated to be a useful tool for molecular classification of tumors.

Experimental design: Using DNA microarrays (CNIO-OncoChip) containing 6386 cancer-related genes, we established the expression profiling of T-cell lymphomas and compared them to normal lymphocytes and lymph nodes.

Results: We found significant differences between the peripheral and lymphoblastic T-cell lymphomas, which include a deregulation of nuclear factor-kappaB signaling pathway. We also identify differentially expressed genes between peripheral T-cell lymphoma tumors and normal T lymphocytes or reactive lymph nodes, which could represent candidate tumor markers of these lymphomas. Additionally, a close relationship between genes associated to survival and those that differentiate among the stages of disease and responses to therapy was found.

Conclusions: Our results reflect the value of gene expression profiling to gain insight about the molecular alterations involved in the pathogenesis of T-cell lymphomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival
  • Cluster Analysis
  • DNA Primers / chemistry
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Lymph Nodes / pathology
  • Lymphatic Metastasis
  • Lymphoma, T-Cell / diagnosis*
  • Lymphoma, T-Cell / metabolism*
  • Lymphoma, T-Cell / pathology
  • Lymphoma, T-Cell, Peripheral / diagnosis*
  • Lymphoma, T-Cell, Peripheral / genetics
  • Lymphoma, T-Cell, Peripheral / metabolism
  • Male
  • Multigene Family
  • NF-kappa B / metabolism
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Phylogeny
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / diagnosis*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • NF-kappa B