Abstract
Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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DNA Primers
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Escherichia coli / classification*
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Escherichia coli / genetics
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Escherichia coli / isolation & purification*
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Escherichia coli Infections / diagnosis
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Genes, Bacterial / genetics
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Geography
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Humans
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Polymerase Chain Reaction / methods*
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Random Amplified Polymorphic DNA Technique / methods