Abstract
Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Southern
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Cell Adhesion / immunology*
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Electrophoresis, Polyacrylamide Gel
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Flow Cytometry
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Humans
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Intercellular Adhesion Molecule-1 / immunology
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Intercellular Adhesion Molecule-1 / metabolism
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Jurkat Cells
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Lymphocyte Function-Associated Antigen-1 / immunology*
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Mutation
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RNA, Messenger / analysis
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Signal Transduction / immunology
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T-Lymphocytes / immunology*
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Transcription Factors / genetics
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Transcription Factors / immunology*
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rho GTP-Binding Proteins / genetics
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rho GTP-Binding Proteins / immunology*
Substances
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Lymphocyte Function-Associated Antigen-1
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RNA, Messenger
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RhoH protein, human
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Transcription Factors
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Intercellular Adhesion Molecule-1
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rho GTP-Binding Proteins