Abstract
We made a transgenic mouse that expresses Cre recombinase activity in inner ear hair cells by using a modified bacterial artificial chromosome containing Prestin. Cre recombinase activity was restricted to inner and outer hair cells, a subset of vestibular hair cells, spiral and vestibular ganglia in the inner ear, and a subset of cells in the testis, epididymis, and ear bone. This mouse will be useful for hair-cell-specific gene targeting.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Chromosomes, Artificial, Bacterial / genetics*
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Epididymis / metabolism
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Gene Targeting
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Hair Cells, Auditory, Inner / metabolism*
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Hair Cells, Auditory, Outer / metabolism*
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Integrases / genetics*
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Male
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Mice
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Mice, Transgenic
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Molecular Motor Proteins
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Proteins / genetics*
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Testis / metabolism
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Vestibular Nerve / metabolism
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Viral Proteins / genetics*
Substances
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Molecular Motor Proteins
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Pres protein, mouse
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Proteins
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Viral Proteins
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Cre recombinase
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Integrases