Microdistributions of prospective BNCT-compound CuTCPH in tissue sections with a heavy ion microbeam

Appl Radiat Isot. 2004 Nov;61(5):771-4. doi: 10.1016/j.apradiso.2004.05.062.

Abstract

Microdistributions of the prospective BNCT-compound CuTCPH, a carborane-containing tetraphenylporphyrin with one Cu atom in its molecular structure, have been obtained in tissue sections of different organs of tumor-bearing and normal Syrian hamsters injected with the boron compound by employing a heavy ion microbeam. High resolution X-ray spectroscopy following micro-PIXE (Particle Induced X-ray Emission with micrometer-sized beams) with a focused (16)O ion beam was used. Focusing was performed with a heavy-ion scanning high-precision magnetic quadrupole triplet microprobe. Squamous Cell Carcinomas were induced on the right Cheek Pouch of Syrian Hamsters (HCP), sampled, cryo-sectioned and freeze-dried. Two-dimensional maps of elemental concentration were obtained by scanning the beam over the samples. Very non-uniform Cu concentrations were found in all sections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Boron Neutron Capture Therapy / instrumentation
  • Boron Neutron Capture Therapy / methods*
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / radiotherapy
  • Cheek
  • Cricetinae
  • Mesocricetus
  • Metalloporphyrins / pharmacokinetics*
  • Metalloporphyrins / therapeutic use*
  • Mouth Neoplasms / metabolism
  • Mouth Neoplasms / radiotherapy
  • Spectrometry, X-Ray Emission
  • Tissue Distribution

Substances

  • Metalloporphyrins
  • copper tetracarboranyltetraphenylporphyrin