Abstract
The gamma delta T cell receptor (TCR) of the hybridoma KN6 recognizes the self molecule encoded by a class I gene which maps within the TL region of the major histocompatibility complex (MHC) of H-2b mice. Mice transgenic (Tg) for this TCR were crossed with mice genetically deficient in beta 2-microglobulin (beta 2m). No mature Tg gamma delta T cells were detected in the thymus or the spleen of the beta 2m- gamma delta Tg mice. We conclude that interaction between the Tg gamma delta TCR and a beta 2m-associated molecule (probably an MHC class I molecule) is required for the generation of mature Tg gamma delta T cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Genetically Modified
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Antibodies, Monoclonal / immunology
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Antigens, CD*
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Antigens, Differentiation / immunology
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Base Sequence
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CD24 Antigen
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Cell Differentiation*
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Crosses, Genetic
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Embryo, Mammalian / immunology
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Gene Expression
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Genotype
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Haplotypes
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Immunohistochemistry
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Membrane Glycoproteins*
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Mice
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Mice, Inbred Strains
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Molecular Sequence Data
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Receptors, Antigen, T-Cell, gamma-delta / genetics*
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Receptors, Antigen, T-Cell, gamma-delta / metabolism
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Spleen / cytology
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T-Lymphocytes / metabolism*
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Thymus Gland / cytology
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Thymus Gland / embryology
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beta 2-Microglobulin / deficiency
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beta 2-Microglobulin / genetics*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation
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CD24 Antigen
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Cd24a protein, mouse
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Membrane Glycoproteins
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Receptors, Antigen, T-Cell, gamma-delta
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beta 2-Microglobulin