Impact of nelfinavir resistance mutations on in vitro phenotype, fitness, and replication capacity of human immunodeficiency virus type 1 with subtype B and C proteases

Luis M F Gonzalez; Rodrigo M Brindeiro; Renato S Aguiar; Helena S Pereira; Celina M Abreu; Marcelo A Soares; Amilcar Tanuri

doi:10.1128/AAC.48.9.3552-3555.2004

Impact of nelfinavir resistance mutations on in vitro phenotype, fitness, and replication capacity of human immunodeficiency virus type 1 with subtype B and C proteases

Antimicrob Agents Chemother. 2004 Sep;48(9):3552-5. doi: 10.1128/AAC.48.9.3552-3555.2004.

Authors

Luis M F Gonzalez¹, Rodrigo M Brindeiro, Renato S Aguiar, Helena S Pereira, Celina M Abreu, Marcelo A Soares, Amilcar Tanuri

Affiliation

¹ Laboratório de Virologia Molecular, Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS, Bloco A, Cidade Universitária, Ilha do Fundão, 21944-970 Rio de Janeiro, RJ, Brazil.

Abstract

Human immunodeficiency virus type 1 subtype B and C proteases were manipulated to contain 90M, 88D, or 89L, and their in vitro biological properties were studied. We showed that D30N has significantly more impact in subtype C than in subtype B counterparts, accounting for the reported low prevalence of this mutation in patients failing nelfinavir-based regimens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cloning, Molecular
DNA Primers
Drug Resistance, Viral
HIV Infections / virology
HIV Protease / genetics*
HIV Protease Inhibitors / pharmacology*
HIV-1 / drug effects*
HIV-1 / enzymology*
HIV-1 / genetics
Humans
Isoenzymes / genetics
Mutation / genetics*
Nelfinavir / pharmacology*
Phenotype
RNA, Viral / genetics
Recombinant Fusion Proteins / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Virus Replication / drug effects

Substances

DNA Primers
HIV Protease Inhibitors
Isoenzymes
RNA, Viral
Recombinant Fusion Proteins
HIV Protease
Nelfinavir