[Anti-SARS virus activities of different recombinant human interferons in cell culture system]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep;17(3):205-8.
[Article in Chinese]

Abstract

Background: To study the anti-SARS virus activities of different recombinant human interferons on the cell culture system.

Methods: Anti-SARS virus activities of interferons were determined by using CPE inhibition test in human skeletal muscle sarcoma (Rda) cell culture.

Results: The average minimum amount of interferon alpha 2b, alpha 1b, beta 1b or omega 1b to inhibit 50% CPE in Rda cell culture was (160.5+/-129.5) IU/ml, (149.0+/-71.7) IU/ml, (69.5+/-61.5) IU/ml, (87.3+/-47.1) IU/ml, respectively or (0.6+/-0.5) ng/ml, (10.6+/-5.1) ng/ml, (3.5+/-3.1) ng/ml, (0.9+/-0.5) ng/ml, respectively.

Conclusion: All the tested recombinant interferons showed anti-SARS virus activities on the Rda cell culture with different sensitivities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Cell Line, Tumor
  • Humans
  • Interferon Type I / pharmacology*
  • Interferon alpha-2
  • Interferon-alpha / pharmacology*
  • Recombinant Proteins
  • Severe Acute Respiratory Syndrome / drug therapy
  • Severe Acute Respiratory Syndrome / virology
  • Severe acute respiratory syndrome-related coronavirus / drug effects*

Substances

  • Antiviral Agents
  • Interferon Type I
  • Interferon alpha-2
  • Interferon-alpha
  • Recombinant Proteins