Abstract
We report on the function of the human ortholog of Saccharomyces cerevisiae Rif1 (Rap1-interacting factor 1). Yeast Rif1 associates with telomeres and regulates their length. In contrast, human Rif1 did not accumulate at functional telomeres, but localized to dysfunctional telomeres and to telomeric DNA clusters in ALT cells, a pattern of telomere association typical of DNA-damage-response factors. After induction of double-strand breaks (DSBs), Rif1 formed foci that colocalized with other DNA-damage-response factors. This response was strictly dependent on ATM (ataxia telangiectasia mutated) and 53BP1, but not affected by diminished function of ATR (ATM- and Rad3-related kinase), BRCA1, Chk2, Nbs1, and Mre11. Rif1 inhibition resulted in radiosensitivity and a defect in the intra-S-phase checkpoint. The S-phase checkpoint phenotype was independent of Nbs1 status, arguing that Rif1 and Nbs1 act in different pathways to inhibit DNA replication after DNA damage. These data reveal that human Rif1 contributes to the ATM-mediated protection against DNA damage and point to a remarkable difference in the primary function of this protein in yeast and mammals.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Ataxia Telangiectasia Mutated Proteins
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Base Sequence
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Carrier Proteins
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Cell Cycle Proteins
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Cells, Cultured
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DNA Damage / physiology
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DNA Replication / radiation effects
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DNA-Binding Proteins / physiology
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Fluorescent Antibody Technique
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Humans
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Immunoblotting
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Intracellular Signaling Peptides and Proteins*
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Phosphoproteins
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Protein Serine-Threonine Kinases / metabolism*
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RNA Interference
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RNA, Small Interfering / genetics
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Repressor Proteins / physiology
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S Phase / physiology*
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Saccharomyces cerevisiae Proteins / physiology
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Telomere / physiology*
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Telomere-Binding Proteins / metabolism*
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Telomere-Binding Proteins / physiology
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Transfection
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Tumor Suppressor Proteins
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Tumor Suppressor p53-Binding Protein 1
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Ultraviolet Rays
Substances
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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Phosphoproteins
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RNA, Small Interfering
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Repressor Proteins
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Rif1 protein, human
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Saccharomyces cerevisiae Proteins
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TP53BP1 protein, human
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Telomere-Binding Proteins
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Tumor Suppressor Proteins
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Tumor Suppressor p53-Binding Protein 1
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RIF1 protein, S cerevisiae
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ATM protein, human
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Ataxia Telangiectasia Mutated Proteins
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Protein Serine-Threonine Kinases