Abstract
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Catalogs as Topic
-
Cloning, Molecular / methods*
-
Crystallography, X-Ray / methods
-
DNA, Complementary / biosynthesis*
-
Databases, Genetic
-
Gene Expression / genetics
-
Gene Library*
-
Genomics / methods*
-
Humans
-
Peptide Fragments / chemistry
-
Peptide Fragments / genetics
-
Predictive Value of Tests
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / genetics
-
Sequence Analysis, DNA / methods
-
Solubility
Substances
-
DNA, Complementary
-
Peptide Fragments
-
Recombinant Proteins