Typing of pneumococcal surface protein A (PspA) in Streptococcus pneumoniae isolated during epidemiological surveillance in Brazil: towards novel pneumococcal protein vaccines

Vaccine. 2004 Sep 28;22(29-30):3890-6. doi: 10.1016/j.vaccine.2004.04.009.

Abstract

Pneumococcal protein vaccine based on pneumococcal surface protein A (PspA) is in development with the potential to offer broad range of protection against different strains. We have investigated the frequency of PspA family 1 (Fam1) and family 2 (Fam2) proteins among Streptococcus pneumoniae recovered from ongoing surveillance in Brazil. Fam1 and Fam2 were expressed in comparable rates among 366 isolates, with the potential coverage of 94.3%. PspA families were not associated to age group or source of isolates. However, considering the significant tendency of increasing prevalence of Fam2 associated to widespread dissemination of the genetically-related resistant strains, the monitoring of the PspA families derived from population-based data may be necessary in the context of vaccine development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Bacterial Proteins / analysis*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Blotting, Western
  • Body Fluids / microbiology
  • Brazil
  • Child
  • Child, Preschool
  • DNA, Bacterial / analysis
  • Humans
  • Infant
  • Middle Aged
  • Nasopharynx / microbiology
  • Penicillin Resistance
  • Pneumococcal Infections / microbiology*
  • Pneumococcal Vaccines / chemistry
  • Pneumococcal Vaccines / immunology*
  • Polymerase Chain Reaction
  • Population Surveillance
  • Serotyping
  • Streptococcus pneumoniae / chemistry
  • Streptococcus pneumoniae / classification
  • Streptococcus pneumoniae / immunology*
  • Streptococcus pneumoniae / isolation & purification*
  • Vaccines, Subunit / chemistry
  • Vaccines, Subunit / immunology

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Pneumococcal Vaccines
  • Vaccines, Subunit
  • pneumococcal surface protein A