Vaccination of C57/BL6 mice with Dobrava hantavirus nucleocapsid protein in Freund's adjuvant induced partial protection against challenge

Jonas Klingström; Irina Maljkovic; Bartek Zuber; Erik Rollman; Anne Kjerrström; Ake Lundkvist

doi:10.1016/j.vaccine.2004.03.045

Vaccination of C57/BL6 mice with Dobrava hantavirus nucleocapsid protein in Freund's adjuvant induced partial protection against challenge

Vaccine. 2004 Sep 28;22(29-30):4029-34. doi: 10.1016/j.vaccine.2004.03.045.

Authors

Jonas Klingström¹, Irina Maljkovic, Bartek Zuber, Erik Rollman, Anne Kjerrström, Ake Lundkvist

Affiliation

¹ Microbiology and Tumor Biology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden.

PMID: 15364453
DOI: 10.1016/j.vaccine.2004.03.045

Abstract

Dobrava hantavirus (DOBV) causes a severe form of hemorrhagic fever with renal syndrome (HFRS) for which there is no therapy or vaccine available. We compared the immunogenicity and protective efficacy of recombinant DOBV nucleocapsid protein (rDOBV N) given with Alum or Freund's as adjuvant, or PBS, in C57/BL6 mice. All mice given Alum or Freund's seroconverted as did 6/8 mice given rDOBV N with PBS. Reciprocal geometric mean total IgG-titers were 5380, 18,100, and 800, respectively, while the mean IgG1/IgG2a ratios were 17.5, 9.25, and 12, respectively. Furthermore, ELIspot assays showed higher levels of IL-4 producing peripheral blood mononuclear cells (PBMCs) in the group given Alum as compared to the other groups. Interestingly, only mice receiving rDOBV N with Freund's adjuvant were protected from challenge (75% protected), indicating that the strong Th2-type of immune response induced by Alum against rDOBV N did not induce protection in mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic
Alum Compounds
Animals
Antibodies, Viral / blood
Disease Models, Animal
Female
Freund's Adjuvant*
Hantavirus Infections / immunology
Hantavirus Infections / prevention & control*
Immunoglobulin G / blood
Interleukin-2 / analysis
Interleukin-4 / analysis
Leukocytes, Mononuclear / immunology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Nucleocapsid Proteins / administration & dosage
Nucleocapsid Proteins / immunology*
Orthohantavirus / immunology*
Recombinant Proteins / administration & dosage
Recombinant Proteins / immunology
Vaccination
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / immunology
Viral Vaccines / administration & dosage
Viral Vaccines / immunology*

Substances

Adjuvants, Immunologic
Alum Compounds
Antibodies, Viral
Immunoglobulin G
Interleukin-2
Nucleocapsid Proteins
Recombinant Proteins
Vaccines, Synthetic
Viral Vaccines
Interleukin-4
aluminum sulfate
Freund's Adjuvant