Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

S M Bruisten; G T Noordhoek; A J C van den Brule; B Duim; C H E Boel; K El-Faouzi; R du Maine; S Mulder; D Luijt; J Schirm

doi:10.1128/JCM.42.9.4332-4334.2004

Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

J Clin Microbiol. 2004 Sep;42(9):4332-4. doi: 10.1128/JCM.42.9.4332-4334.2004.

Authors

S M Bruisten¹, G T Noordhoek, A J C van den Brule, B Duim, C H E Boel, K El-Faouzi, R du Maine, S Mulder, D Luijt, J Schirm

Affiliation

¹ GG&GD Municipal Health Service, Public Health Laboratory, Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands. [email protected]

Abstract

The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Outer Membrane Proteins / genetics*
Humans
Neisseria gonorrhoeae / genetics
Neisseria gonorrhoeae / isolation & purification*
Polymerase Chain Reaction / methods*
Reproducibility of Results

Substances

Bacterial Outer Membrane Proteins
cppB protein, Neisseria gonorrhoeae