Abstract
Objective:
Evaluation of the use of short probes as a detection system in real-time PCR.
Design and method:
Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum.
Results:
The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal.
Conclusion:
Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR.
MeSH terms
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Chromosomes, Human, Y
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DNA Probes / chemistry*
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DNA Probes / genetics
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DNA-Binding Proteins / genetics*
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Female
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Humans
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Male
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Nuclear Proteins / genetics*
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Nucleic Acid Hybridization
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Oligonucleotides
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Oligonucleotides, Antisense / chemistry*
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Oligonucleotides, Antisense / genetics
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Plasma / metabolism*
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Polymerase Chain Reaction / methods*
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Pregnancy
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Reproducibility of Results
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Sensitivity and Specificity
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Sex Determination Processes
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Sex-Determining Region Y Protein
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Transcription Factors / genetics*
Substances
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DNA Probes
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DNA-Binding Proteins
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Nuclear Proteins
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Oligonucleotides
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Oligonucleotides, Antisense
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Sex-Determining Region Y Protein
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Transcription Factors
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locked nucleic acid