The association of antibodies to cardiolipin, beta 2-glycoprotein I, prothrombin, and oxidized low-density lipoprotein with thrombosis in 292 patients with familial and sporadic systemic lupus erythematosus

Scand J Rheumatol. 2004;33(4):246-52. doi: 10.1080/03009740410005386.

Abstract

Objective: To determine the prevalence of antibodies to phospholipid-binding plasma proteins (aPL) and to oxidized low-density lipoprotein (OX-LDL), and to study the association of these antibodies with thrombosis and coronary heart disease (CHD) in patients with systemic lupus erythematosus (SLE).

Methods: Clinical data and sera from 89 Finnish patients with familial and 203 with sporadic SLE were available for the study. Enzyme-linked immunosorbent assays (ELISA) were used for antibody determination.

Results: The occurrence of thrombosis in our SLE patients was 13.7% (40/292) and of clinically diagnosed CHD was 1.4% (4/292). All antibody assays, except IgM-aCL, were significantly associated with thrombosis. IgG-aCL alone or in combination with anti beta 2-GPI or with anti OX-LDL were reasonably sensitive (38%, 48%, and 58%, respectively) and specific (87%, 80% and 72%, respectively) for a history of thrombosis. A high risk of arterial thrombosis (TIA or stroke) was associated with positivity of IgG-aCL, anti beta 2-GPI, and anti-prothrombin. Venous thrombosis was significantly associated with all other assays except IgM-aCL and anti-prothrombin. No test correlated with CHD, but the number of affected patients was small. There were three multiplex SLE families with two patients having a history of thrombosis: no consistent pattern of aPL or anti OX-LDL was found in these patients.

Conclusion: IgG-aCL alone or in combination with anti beta 2-GPI or anti OX-LDL are sensitive and specific tests for detecting SLE patients at increased risk of thrombosis. The aetiopathogenesis of thrombosis in familial SLE appears to be multifactorial.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Anticoagulants / analysis
  • Anticoagulants / immunology*
  • Cardiolipins / analysis
  • Cardiolipins / immunology*
  • Cohort Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Finland
  • Glycoproteins / analysis
  • Glycoproteins / immunology*
  • Humans
  • Immunoglobulin G / analysis*
  • Immunoglobulin M / analysis*
  • Lipoproteins, LDL / analysis
  • Lipoproteins, LDL / immunology*
  • Lupus Erythematosus, Systemic / genetics*
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Oxidation-Reduction
  • Predictive Value of Tests
  • Prothrombin / analysis
  • Prothrombin / immunology*
  • Risk Factors
  • Sensitivity and Specificity
  • Thrombosis / etiology*
  • beta 2-Glycoprotein I

Substances

  • Anticoagulants
  • Cardiolipins
  • Glycoproteins
  • Immunoglobulin G
  • Immunoglobulin M
  • Lipoproteins, LDL
  • beta 2-Glycoprotein I
  • oxidized low density lipoprotein
  • Prothrombin