Abstract
In a screen for binding partners of the Epstein-Barr virus transformation-related protein EBNA2, we cloned a novel, evolutionarily conserved protein showing similarity to the Drosophila Parallel Sister Chromatids Protein (PASC). We have named this protein "Friend of EBNA2" (FOE). Human FOE encodes a protein of 1227 amino acids with a functional bipartite nuclear localization signal, an arginine-rich motif, a putative nuclear export signal as well as with three highly acidic regions and a predicted coiled-coil domain. FOE and EBNA2 coimmunoprecipitate from lymphocyte nuclear extracts. RNA and protein blots show that FOE is expressed in all human tissues. FOE is a nuclear protein with the bulk of the protein associated with the insoluble nuclear fraction biochemically defined as the nuclear matrix. Indirect immunofluorescence and dynamic imaging studies suggest that FOE associates with transcriptionally active nuclear subregions in interphase cells and concentrates at the ends of formed chromosomes during mitosis.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence / genetics
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Animals
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Base Sequence / genetics
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COS Cells
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Chromatin / genetics
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Chromatin / metabolism*
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Cloning, Molecular
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Epstein-Barr Virus Nuclear Antigens / genetics
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Epstein-Barr Virus Nuclear Antigens / metabolism*
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HeLa Cells
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / immunology*
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Humans
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Jurkat Cells
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Lymphocytes / immunology
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Mitosis / genetics
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Molecular Sequence Data
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Nuclear Matrix / genetics
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Nuclear Matrix / metabolism
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Nuclear Matrix-Associated Proteins / genetics
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Nuclear Matrix-Associated Proteins / isolation & purification*
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Nuclear Matrix-Associated Proteins / metabolism
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Nuclear Proteins
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Oncogene Proteins / genetics
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Oncogene Proteins / isolation & purification
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Oncogene Proteins / metabolism*
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Protein Structure, Tertiary / genetics
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Proto-Oncogene Proteins
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RNA, Messenger / metabolism
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Signal Transduction / genetics
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Transcriptional Activation / genetics
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Viral Proteins
Substances
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Carrier Proteins
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Chromatin
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DNA, Complementary
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EBNA-2 protein, Human herpesvirus 4
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Epstein-Barr Virus Nuclear Antigens
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Nuclear Matrix-Associated Proteins
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Nuclear Proteins
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Oncogene Proteins
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Proto-Oncogene Proteins
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RNA, Messenger
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Viral Proteins
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WAPL protein, human