Abstract
Bioassay-guided fractionation of the EtOAc-soluble extract of Dendrobium moniliforme afforded a new phenanthraquinone-type metabolite, 7-hydroxy-5,6-dimethoxy-1,4-phenanthrenequinone ( 1), along with the previously reported 5-hydroxy-3,7-dimethoxy-1,4-phenanthrenequinone ( 2). The structures of the compounds were identified mainly on the basis of MS and NMR data. Compound 1 inhibited VHR dual-specificity protein tyrosine phosphatase (DS-PTPase) activity in a dose-dependent manner, displaying an IC (50) value of 3.0 +/- 0.2 microM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Dendrobium*
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Dose-Response Relationship, Drug
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Dual Specificity Phosphatase 3
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Enzyme Inhibitors / administration & dosage
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / pharmacology*
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Enzyme Inhibitors / therapeutic use
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Humans
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Inhibitory Concentration 50
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Plant Stems
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Protein Tyrosine Phosphatases / antagonists & inhibitors*
Substances
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Enzyme Inhibitors
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Plant Extracts
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DUSP3 protein, human
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Dual Specificity Phosphatase 3
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Protein Tyrosine Phosphatases