Abstract
We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Fusarium / cytology
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Fusarium / genetics
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Fusarium / pathogenicity
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Molecular Sequence Data
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Molecular Structure
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Plant Proteins / classification
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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RNA, Viral / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribonucleases / classification
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Ribonucleases / genetics
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Ribonucleases / metabolism*
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Spores, Fungal / metabolism
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Triticum / enzymology*
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Triticum / microbiology*
Substances
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Plant Proteins
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RNA, Viral
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Recombinant Fusion Proteins
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Win-1 proteins, plant
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Ribonucleases