Abstract
Electrochemical DNA detection systems are an attractive approach to the development of multiplexed, high-throughput DNA analysis systems for clinical and research applications. We have engineered a new class of nanoelectrode ensembles (NEEs) that constitute a useful platform for biomolecular electrochemical sensing. High-sensitivity DNA detection was achieved at oligonucleotide-functionalized NEEs using a label-free electrocatalytic assay. Attomole levels of DNA were detected using the NEEs, validating the promise of nanoarchitectures for ultrasensitive biosensing.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Biosensing Techniques / methods
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Catalysis
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Cations / chemistry
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DNA / analysis*
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DNA / chemistry
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DNA / genetics
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Electrochemistry / methods
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Ferric Compounds / chemistry
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Gold / chemistry
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Helicobacter pylori / genetics
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Microelectrodes*
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Microscopy, Electron, Scanning
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Nanotechnology / methods*
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Nucleic Acid Hybridization
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Oligonucleotides / chemistry
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Oligonucleotides / genetics
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RNA, Ribosomal, 23S / analysis
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RNA, Ribosomal, 23S / genetics
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Ruthenium / chemistry
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Sensitivity and Specificity
Substances
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Cations
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Ferric Compounds
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Oligonucleotides
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RNA, Ribosomal, 23S
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Gold
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Ruthenium
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DNA