Abstract
Technical advances in the field of live-cell imaging have introduced the cell biologist to a new, dynamic, subcellular world. The static world of molecules in fixed cells has now been extended to the time dimension. This allows the visualization and quantification of gene expression and intracellular trafficking events of the studied molecules and the associated enzymatic processes in individual cells, in real time.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Cells, Cultured
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DNA-Binding Proteins / metabolism
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Diagnostic Imaging*
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Gene Expression Profiling*
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Gene Expression Regulation*
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Microscopy / instrumentation
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Microscopy / methods
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Proteins / metabolism
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RNA, Messenger / metabolism
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Repressor Proteins / metabolism
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Staining and Labeling / methods
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Time Factors
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Transcription Factors / metabolism
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Yeasts / cytology
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Yeasts / metabolism
Substances
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DNA-Binding Proteins
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Proteins
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RNA, Messenger
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Repressor Proteins
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Transcription Factors