Abstract
The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Azospirillum brasilense / drug effects
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Azospirillum brasilense / genetics*
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Azospirillum brasilense / metabolism*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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DNA, Bacterial / genetics
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Genes, Bacterial
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Molecular Sequence Data
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Mutation
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Nitrates / metabolism
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Nitrogen Fixation / genetics
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Nitrogenase / genetics
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Nitrogenase / metabolism
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Nitrosoguanidines / pharmacology
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Operon
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PII Nitrogen Regulatory Proteins
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Phenotype
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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DNA, Bacterial
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Nitrates
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Nitrosoguanidines
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PII Nitrogen Regulatory Proteins
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Repressor Proteins
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PIID regulatory protein, Bacteria
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Nitrogenase