Effects of eplerenone, a selective aldosterone blocker, on the progression of left ventricular dysfunction and remodeling in rats with dilated cardiomyopathy

Pharmacology. 2005 Feb;73(2):81-8. doi: 10.1159/000081267. Epub 2004 Oct 1.

Abstract

Aldosterone blockade reduces morbidity and mortality in patients with heart failure. We studied the effects of eplerenone, a novel aldosterone blocker, on the progression of left ventricular dysfunction and remodeling in rats with dilated cardiomyopathy after autoimmune myocarditis. Twenty-eight days after immunization, the surviving Lewis rats were randomized to 1 month's oral treatment with low-dose eplerenone (group L), high-dose eplerenone (group H) or vehicle (group V). Five of 15 (33%) rats in group V and 3 of 15 (20%) rats in group L died during the course of treatment. High-dose eplerenone significantly reduced cardiomyocyte hypertrophy, heart weight and heart weight to body weight ratio. Eplerenone improved left ventricular function in a dose-dependent manner. Central venous pressure and left ventricular end-diastolic pressure were lower, and +/-dP/dt and fractional shortening were higher in group H than group V. Eplerenone also attenuated myocardial fibrosis and reduced left ventricular mRNA expressions of TGF-beta(1) and collagen-III. Our results indicate that treatment with eplerenone improved left ventricular dysfunction and attenuated left ventricular remodeling in rats with heart failure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoimmune Diseases / complications
  • Cardiomyopathy, Dilated / etiology
  • Cardiomyopathy, Dilated / pathology
  • Cardiomyopathy, Dilated / physiopathology*
  • Collagen Type III / biosynthesis
  • Collagen Type III / genetics
  • Dose-Response Relationship, Drug
  • Electrocardiography
  • Eplerenone
  • Fibrosis
  • Hemodynamics / drug effects
  • Male
  • Mineralocorticoid Receptor Antagonists*
  • Myocarditis / complications
  • Myocardium / metabolism
  • Myocardium / pathology
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Inbred Lew
  • Spironolactone / analogs & derivatives*
  • Spironolactone / pharmacology*
  • Transforming Growth Factor beta / biosynthesis
  • Transforming Growth Factor beta / genetics
  • Ventricular Dysfunction, Left / drug therapy*
  • Ventricular Dysfunction, Left / etiology
  • Ventricular Dysfunction, Left / physiopathology
  • Ventricular Remodeling / drug effects*

Substances

  • Collagen Type III
  • Mineralocorticoid Receptor Antagonists
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Spironolactone
  • Eplerenone