Abstract
Phytochemical investigation of the aqueous extract of the roots of Agrimonia pilosa Ledeb. (Rosaceae), as guided by hepatoprotective activity in vitro, furnished two isocoumarins, agrimonolide (1) and agrimonolide 6-O-beta-D-glucoside (3), and (+)-catechin (2). Compound 1 showed hepatoprotective effects on both tacrine-induced cytotoxicity in human liver-derived Hep G2 cells and tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes with EC50 values of 88.2 +/- 2.8 and 37.7 +/- 1.6 microM, respectively.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Agrimonia*
-
Animals
-
Cell Line, Tumor
-
Coumarins / chemistry
-
Coumarins / isolation & purification*
-
Coumarins / pharmacology*
-
Hepatocytes / drug effects*
-
Hepatocytes / physiology
-
Humans
-
Male
-
Plant Extracts / chemistry
-
Plant Extracts / isolation & purification
-
Plant Extracts / pharmacology
-
Plant Roots
-
Rats
-
Rats, Sprague-Dawley
-
Tacrine / toxicity
-
tert-Butylhydroperoxide / toxicity
Substances
-
Coumarins
-
Plant Extracts
-
Tacrine
-
tert-Butylhydroperoxide