The structure of the O-polysaccharide from the lipopolysaccharide of Providencia stuartii O47

Carbohydr Res. 2004 Oct 20;339(15):2621-6. doi: 10.1016/j.carres.2004.08.011.

Abstract

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O47:H4, strain 3646/51. Studies by sugar and methylation analyses along with Smith degradation and 1H and 13C NMR spectroscopy, including two-dimensional 1H,1H COSY, TOCSY, ROESY and H-detected 1H,13C HSQC and HMBC experiments, showed that the polysaccharide has a branched hexasaccharide repeating unit with the following structure: [carbohydrate structure: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • O Antigens / chemistry*
  • Providencia / chemistry*
  • Providencia / immunology

Substances

  • Lipopolysaccharides
  • O Antigens