Cloning and characterization of a gene encoding extracellular metalloprotease from Streptomyces lividans

Gene. 1992 Feb 1;111(1):125-30. doi: 10.1016/0378-1119(92)90613-t.

Abstract

The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200 micrograms/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phenanthroline or EDTA. The region upstream from prt codes for an incomplete open reading frame (ORF) oriented opposite to prt. This ORF has a strong similarity to a gene family (lysR) whose members regulate the transcription of structural genes required for either biosynthesis or degradation.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Bacterial*
  • Genomic Library
  • Hydrogen-Ion Concentration
  • Metalloendopeptidases / genetics*
  • Metalloendopeptidases / metabolism
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Alignment
  • Streptomyces / enzymology
  • Streptomyces / genetics*
  • Temperature

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Metalloendopeptidases
  • Prt protein, Streptomyces

Associated data

  • GENBANK/M64367
  • GENBANK/M64425
  • GENBANK/M64426
  • GENBANK/M64427
  • GENBANK/M89476
  • GENBANK/S39996
  • GENBANK/S40001
  • GENBANK/S40008
  • GENBANK/S40009
  • GENBANK/S40010