An interclass nuclear transfer between fowl and mammal: in vitro development of chicken-to-cattle interclass embryos and the detection of chicken genetic complements

Tae M Kim; Tae S Park; Sang S Shin; Jae Y Han; Shin Y Moon; Jeong M Lim

doi:10.1016/j.fertnstert.2004.06.019

An interclass nuclear transfer between fowl and mammal: in vitro development of chicken-to-cattle interclass embryos and the detection of chicken genetic complements

Fertil Steril. 2004 Oct;82(4):957-9. doi: 10.1016/j.fertnstert.2004.06.019.

Authors

Tae M Kim¹, Tae S Park, Sang S Shin, Jae Y Han, Shin Y Moon, Jeong M Lim

Affiliation

¹ Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.

PMID: 15482780
DOI: 10.1016/j.fertnstert.2004.06.019

Abstract

An attempt was made to develop an interclass somatic cell nuclear transfer method as an alternative means of establishing chicken embryonic stem cells. Chicken-to-cattle interclass embryos that activated calcium ionophore, cycloheximide, and cytochalasin D were developed into blastocysts, and the developing interclass embryos had chicken genetic complements.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle / embryology*
Cattle / genetics
Cell Fusion / methods*
Cell Nucleus / genetics
Chick Embryo / embryology*
Female
Gene Transfer, Horizontal / genetics*
Nuclear Transfer Techniques*
Oocytes / cytology*
Stem Cells / cytology