Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K

Bioresour Technol. 2005 Mar;96(4):429-36. doi: 10.1016/j.biortech.2004.06.012.

Abstract

The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degrees C with 37 degrees C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioreactors / microbiology*
  • Cell Culture Techniques / methods*
  • Cell Proliferation
  • Drug Tolerance
  • Enzyme Activation
  • Enzyme Stability
  • Hydrogen-Ion Concentration
  • Motion
  • Organic Chemicals / chemistry*
  • Peptide Hydrolases / biosynthesis*
  • Peptide Hydrolases / chemistry*
  • Peptide Hydrolases / classification
  • Pseudomonas aeruginosa / classification*
  • Pseudomonas aeruginosa / enzymology*
  • Solvents / chemistry*
  • Species Specificity
  • Temperature

Substances

  • Organic Chemicals
  • Solvents
  • Peptide Hydrolases