Abstract
A substantial number of G-protein-coupled receptor-interacting proteins have been identified initially by the use of yeast two-hybrid screens. Using the C-terminal tail of both opioid receptors and the melanin concentrating hormone receptor-1 as bait, the actin and intermediate filament-binding protein periplakin was isolated. In each case, the site of interaction is within helix VIII of the receptor and periplakin limits agonist-mediated G-protein activation potentially by competing with G-protein for this region of the receptor.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Allosteric Regulation
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Animals
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Cytoskeletal Proteins / chemistry*
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Cytoskeletal Proteins / metabolism
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GTP-Binding Proteins / chemistry*
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Humans
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Plakins
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Protein Binding
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Protein Conformation
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Protein Structure, Secondary
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Receptors, G-Protein-Coupled / chemistry*
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Receptors, G-Protein-Coupled / metabolism
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Receptors, Melanocortin / chemistry
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Two-Hybrid System Techniques
Substances
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Cytoskeletal Proteins
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Plakins
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Ppl protein, mouse
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Receptors, G-Protein-Coupled
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Receptors, Melanocortin
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GTP-Binding Proteins