Modulation of transcription affects mRNP quality

Mol Cell. 2004 Oct 22;16(2):235-44. doi: 10.1016/j.molcel.2004.09.019.

Abstract

Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/TREX complex and the mRNA termination/polyadenylation apparatus. Here we demonstrate a prominent role for the rate of transcription in the production of export-competent mRNPs. We show that a transcription-defective allele of the Rad3p helicase, a component of the TFIIH transcription initiation factor, suppresses several phenotypes associated with defective mRNA processing and export. Strikingly, the effects of compromised Rad3p activity can be phenocopied by a transcription elongation drug as well as by other mutations affecting transcription. Our results suggest that efficient mRNP assembly is under a kinetic control that is influenced by the rate of transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism
  • DNA Helicases / genetics
  • DNA Helicases / metabolism
  • Gene Expression Regulation / physiology*
  • Mutation
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / metabolism*
  • Saccharomyces cerevisiae Proteins
  • Transcription, Genetic / physiology*
  • Yeasts / genetics
  • Yeasts / physiology

Substances

  • RNA, Messenger
  • Ribonucleoproteins
  • Saccharomyces cerevisiae Proteins
  • messenger ribonucleoprotein
  • Adenosine Triphosphatases
  • Rad3 protein, S cerevisiae
  • DNA Helicases