Abstract
The neuro-endocrine X-organ sinus-gland complex of crustaceans produces and releases the neuropeptides of the crustacean hyperglycemic hormone (cHH)/molt-inhibiting hormone (MIH)/gonad-inhibiting hormone (GIH) family that regulate important physiological processes, such as growth, reproduction and molting. We cloned two full-length cDNAs encoding the preprocHH-A and preprocHH-B of the Norway lobster Nephrops norvegicus of 132 and 131 amino acid residues. The two cHHs differ in the preprohormone but not in the mature peptide sequence. The mature cHH was expressed in bacteria as GST fusion protein that, in bioassay, shows a hyperglycemic activity similar to that of native cHH present in an eyestalk extract.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Arthropod Proteins
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Base Sequence
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Biological Assay
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Blotting, Western
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Cloning, Molecular
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Conserved Sequence
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression
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Glutathione Transferase / metabolism
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Invertebrate Hormones / chemistry*
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Invertebrate Hormones / metabolism
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Molecular Sequence Data
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Nephropidae / genetics*
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Nerve Tissue Proteins / chemistry*
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Nerve Tissue Proteins / metabolism
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Neuropeptides / genetics
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Neuropeptides / metabolism
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Polymerase Chain Reaction
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Protein Precursors / chemistry*
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Protein Precursors / metabolism
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RNA, Messenger / analysis
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
Substances
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Arthropod Proteins
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DNA, Complementary
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Invertebrate Hormones
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Nerve Tissue Proteins
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Neuropeptides
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Protein Precursors
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RNA, Messenger
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Recombinant Fusion Proteins
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hyperglycemic hormone, crustacean
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hyperglycemic hormone precursor, crustacean
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Glutathione Transferase