Cleavage of the N-linked oligosaccharide from the surfaces of Chlamydia species affects attachment and infectivity of the organisms in human epithelial and endothelial cells

Infect Immun. 2004 Nov;72(11):6699-701. doi: 10.1128/IAI.72.11.6699-6701.2004.

Abstract

Previous studies demonstrated that the high-mannose oligosaccharide N linked to the Chlamydia major outer membrane protein inhibited the attachment and infectivity of the organism. The present study showed that cleavage of the glycan with N-glycanase decreased the attachment and infectivity of chlamydial organisms in human epithelial and endothelial cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Adhesion*
  • Cell Line
  • Chlamydia / classification
  • Chlamydia / physiology*
  • Endothelial Cells / microbiology*
  • Epithelial Cells / microbiology*
  • Glycoside Hydrolases / metabolism*
  • Humans
  • Oligosaccharides / metabolism*
  • Polysaccharides / metabolism

Substances

  • Oligosaccharides
  • Polysaccharides
  • Glycoside Hydrolases
  • glycanase