T-cell properties determine disease site, clinical presentation, and cellular pathology of experimental autoimmune encephalomyelitis

Am J Pathol. 2004 Nov;165(5):1519-33. doi: 10.1016/S0002-9440(10)63410-4.

Abstract

Two distinct clinical phenotypes of experimental autoimmune encephalomyelitis are observed in BALB interferon-gamma knockout mice immunized with encephalitogenic peptides of myelin basic protein. Conventional disease, characterized by ascending weakness and paralysis, occurs with greater frequency after immunizing with a peptide comprising residues 59 to 76. Axial-rotatory disease, characterized by uncontrolled axial rotation, occurs with greater frequency in mice immunized with a peptide corresponding to exon 2 of the full length 21.5-kd protein. The two clinical phenotypes are histologically distinguishable. Conventional disease is characterized by inflammation and demyelination primarily in spinal cord, whereas axial-rotatory disease involves inflammation and demyelination of lateral medullary areas of brain. Both types have infiltrates in which neutrophils are a predominating component. By isolating T cells and transferring disease to naive recipients, we show here that the type of disease is determined entirely by the inducing T cell. Furthermore, studies using CXCR2 knockout recipients, unable to recruit neutrophils to inflammatory sites, show that although neutrophils are critical for some of these T cells to effect disease, there are also interferon-gamma-deficient T cells that induce disease in the absence of both interferon-gamma and neutrophils. These results highlight the multiplicity of T-cell-initiated effector pathways available for inflammation and demyelination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation
  • Cell Proliferation
  • Central Nervous System / cytology
  • Cloning, Molecular
  • Cytokines / metabolism
  • Encephalomyelitis, Autoimmune, Experimental / blood*
  • Enzyme-Linked Immunosorbent Assay
  • Exons
  • Flow Cytometry
  • Heterozygote
  • Homozygote
  • Inflammation
  • Interferon-gamma / genetics
  • Interferon-gamma / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Myelin Basic Protein / physiology
  • Neutrophils / metabolism
  • Peptides / chemistry
  • Phenotype
  • Protein Structure, Tertiary
  • Ribonucleases / metabolism
  • T-Lymphocytes / pathology*
  • Time Factors

Substances

  • Cytokines
  • Myelin Basic Protein
  • Peptides
  • Interferon-gamma
  • Ribonucleases