Abstract
The amplification product obtained with DNA from vancomycin-resistant (VmR) Enterococcus gallinarum BM4174 and a pair of degenerate oligodeoxyribonucleotides that correspond to conserved amino acid (aa) motifs in Escherichia coli D-alanine (D-Ala):D-Ala ligases and in En. faecium VmR protein (VanA) was used as a probe to clone the vanC gene of that strain. The vanC product, with a calculated Mr of 37,504, exhibits 29 to 38% aa identity with VanA and E. coli ligases. Insertional inactivation of vanC led to Vm sensitivity of BM4174 suggesting that the gene may encode a D-Ala:D-Ala ligase of altered specificity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Blotting, Southern
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DNA Mutational Analysis
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Drug Resistance / genetics
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Enterococcus / drug effects
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Enterococcus / genetics*
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Genes, Bacterial
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Molecular Sequence Data
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Peptide Synthases / chemistry
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Peptide Synthases / genetics*
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Vancomycin / pharmacology*
Substances
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Bacterial Proteins
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Vancomycin
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Peptide Synthases
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D-alanylalanine synthetase
Associated data
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GENBANK/M75132
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GENBANK/M76680
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GENBANK/M76682
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GENBANK/M79307
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GENBANK/M79308
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GENBANK/M79309
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GENBANK/M79310
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GENBANK/X13839
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GENBANK/X60732
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GENBANK/X60733