Abstract
A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and heterotrophic bacteria.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / chemistry*
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Bacteria / classification*
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Bacteria / genetics
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Bacteria / growth & development
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Bacterial Typing Techniques
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Biomarkers
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Biomass
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DNA, Ribosomal / analysis
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Ecosystem
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Electrophoresis, Polyacrylamide Gel / methods*
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Fresh Water / microbiology
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Genes, rRNA
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Molecular Sequence Data
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Phospholipids / analysis*
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RNA, Ribosomal, 16S / genetics*
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Sequence Analysis, DNA
Substances
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Biomarkers
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DNA, Ribosomal
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Phospholipids
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RNA, Ribosomal, 16S
Associated data
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GENBANK/AY525644
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GENBANK/AY525645
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GENBANK/AY525646
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GENBANK/AY525647
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GENBANK/AY525648
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GENBANK/AY525649
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GENBANK/AY525650
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GENBANK/AY525651
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GENBANK/AY525652
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GENBANK/AY525653
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GENBANK/AY525654
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GENBANK/AY525655
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GENBANK/AY525656
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GENBANK/AY525657
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GENBANK/AY525658
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GENBANK/AY525659
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GENBANK/AY525660
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GENBANK/AY525661
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GENBANK/AY525662
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GENBANK/AY525663
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GENBANK/AY525664
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GENBANK/AY525665
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GENBANK/AY525666
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GENBANK/AY525667
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GENBANK/AY525668
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GENBANK/AY525669
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GENBANK/AY525670
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GENBANK/AY525671
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GENBANK/AY525672